International Journal of Applied Biology
Vol. 5 No. 2 (2021): International Journal of Applied Biology

Seperation of bioactive compounds from Haemolymph of scarab beetle Scarabaeus sacer (Coleoptera: Scarabaeidae) by GC-MS and determination of its antimicrobial activity

doaa hassan abdelsalam (a:1:{s:5:"en_US"
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Nancy Taha Mohamed (professor of insect physiology, faculty of science, helwan university)



Article Info

Publish Date
31 Dec 2021

Abstract

This study aimed to investigate the bioactive compounds in the haemolymph of scarab beetle Scarabaeus sacer by using the Gas chromatography–mass spectrometry (GS-MS) analysis. The identification of the bioactive compounds is based on peak area, retention time, molecular formula and molecular weight. There are 129 compounds are detected in the haemolymph of scarab beetle and 43 of them were reported to have a bioactivity. The most analyzed bioactive compounds are alcohols, steroids, fatty acids and terpenoids. The current study also test the antimicrobial activity of scarab beetle haemolymph against gram-negative bacteria (Escherichia coli, Enterobacter cloacae, gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis), and fungi (Aspergillus fumigatus and Candida albicans). The haemolymph has highest antibacterial activity against gram negative bacteria Enterobacter cloacae, Escherichia coli respectively and against gram-positive bacteria Bacillus subtilis, Staphylococcus aureus respectively. No antifungal activity has been detected.

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Journal Info

Abbrev

ijoab

Publisher

Subject

Agriculture, Biological Sciences & Forestry Automotive Engineering Energy Environmental Science

Description

International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. ...