Jurnal Bahan Alam Terbarukan
Vol 6, No 2 (2017): December 2017 [Nationally Accredited]

BIODEGRADATION OF CHRYSENE BY CONSORTIUM OF BACILLUS CEREUS AND PSEUDOMONAS PUTIDA IN PETROLEUM CONTAMINATED-SOIL ON SLURRY-PHASE BIOREACTOR




Article Info

Publish Date
21 Dec 2017

Abstract

Pollution by chrysene compounds in the polluted soil of petroleum, due to exploration activities, production and disposal of petroleum waste into the environment causing serious damage to the ecosystem environment, became the target of processing with bacteria as a model of remediation of pollution sites. Thus, the study focused on the use of a bacterial consortium to degrade chrysene in petroleum-contaminated soil. The study was conducted by mixing 20:80 (% wt) of contaminated soil with water. The consortium of Bacillus cereus and Pseudomonas putida 10%(v/v) and 15%(v/v) bacteria with ratios; 2:3; 1:1; 3:2 is inserted into the slurry bioreactor. Biodegradation process is run with agitation of 100 rpm and temperature 26supo/supC – 30supo/supC and in aeration. Identification of chrysene using gas chromatography–mass spectrometry (GCMS) and bacterial populations with haemycitometer. The initial concentration of chrysene is 24.48 ng/μL. After 49 days remediation period for a 10% (v/v) reduced chrysene bacteria consortium and bacterial populations were 8.68 ng/μL; 7.56 ng/μL; and 8.07 ng/μL; with biodegradation rate is 67.01%; 69.10%; And 64.54%. As for the 15% (v/v) bacteria consortium with the same ratio, chrysene was degraded to 2.60 ng/μL; 1.57 ng/μL; and 2.02 ng/μL and the measured chrysene biodegradation rate was 89.39%; 93.58%; And 91.73%. These findings suggest that the percentage of low crude oil is degraded because of the increasing concentration of crude oil.

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Journal Info

Abbrev

jbat

Publisher

Subject

Materials Science & Nanotechnology

Description

This journal presents articles and information on research, development and applications in biomass conversion processes (thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion) and equipment to produce fuels, power, heat, and value-added chemicals from biomass. A ...