Jurnal Riset Kimia
Vol. 9 No. 1 (2015): September

PEROLEHAN GLUKOSA DENGAN HIDROLISIS ENZIMATIS DARI AMPAS TEBU MENGGUNAKAN Trichoderma viride DAN Aspergilus niger SEBAGAI BAHAN BAKU BIOETANOL

Elita Sari (Teknik Kimia Fakultas Teknologi Industri Universitas Bung Hatta Padang Jl. Gajah Mada 19 Gunung Pangilun Padang)
Elly Desni Rahman (Teknik Kimia Fakultas Teknologi Industri Universitas Bung Hatta Padang Jl. Gajah Mada 19 Gunung Pangilun Padang)
Munas Martynis (Teknik Kimia Fakultas Teknologi Industri Universitas Bung Hatta Padang Jl. Gajah Mada 19 Gunung Pangilun Padang)
Shafira Fiona (Teknik Kimia Fakultas Teknologi Industri Universitas Bung Hatta Padang Jl. Gajah Mada 19 Gunung Pangilun Padang)
Junialdi - (Teknik Kimia Fakultas Teknologi Industri Universitas Bung Hatta Padang Jl. Gajah Mada 19 Gunung Pangilun Padang)



Article Info

Publish Date
05 Apr 2015

Abstract

Lignocellulosic biomass is a rich agricultural waste containing cellulose and hemicellulose. Bagasse (bagasse) is one of the waste biomass containing cellulose and hemicellulose approximately 47.7% which can be used to produce bioethanol. Enzymatic process is able to hydrolysis the polysaccharide compound into its constituent, sugars monomer compound. An enzymatic hydrolysis process converts cellulose to glucose by using cellulase enzymes. Some microorganisms producing the enzyme cellulase are Trichoderma viride and Aspergillus niger. The purpose of this research is to find out comparison of both these microorganism as a catalyst in the enzymatic hydrolysis process that produces the highest glucose levels and hydrolysis time. Hydrolysis process is done by varying the ratio between Tricoderma viride and Aspergillus niger of 1: 0; 0: 1; 0.5: 1 and 1: 0.5 and  time sampling  24, 48, 72, 96, 120. The results showed glucose acquisition as much as 360 mg/L with hidrolysis process  by hotplate  stirer and 660 mg/L with process by waterbath shaker at composition ratio of Trichoderma viride: Aspergillus niger 1: 0.5 and the hydrolysis time is 96 hours.

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