Screening of Actinomycetes Producing an ATPase Inhibitor of Japanese Encephalitis Virus RNA Helicase from Soil and Leaf Litter Samples

SHANTI RATNAKOMALA
RONI RIDWAN (Research Center for Biotechnology LIPI)
PUSPITA LISDIYANTI (Research Center for Biotechnology LIPI)
. ABINAWANTO (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia)
UTAMA ANDI (Research Center for Biotechnology LIPI)

Microbiology Indonesia
Vol. 5 No. 1 (2011): March 2011

Actinomycetes are commercially important microorganisms for the production of antibiotics, enzymes, inhibitors of enzymes, and other bioactive secondary metabolites. Some 853 isolates of actinomycetes were isolated from soil and leaf litter samples in Kupang NTT and Enrekang, South Sulawesi. Those isolates were then tested for inhibition of ATPase activity of RNA helicase from Japanese encephalitis virus (JEV), in order to identify a drug candidate for the treatment of JEV infection. Results revealed that 14 isolates have relatively high inhibition-activity on JEV ATPase activity of the JEV-RNA-helicase, which range from approximately 40.0-50.0% inhibition. The highest inhibition-activity was identified in Actinoplanes philippinensis 5-849 with 49. 9% of inhibition-activity and Streptomyces chartreusis 5-095 with 49.2% of inhibition-activity