Microbiology Indonesia
Vol. 5 No. 3 (2011): September 2011

Isolation and Characterization of Chitinolytic Bacteria and Their Potential to Inhibit Plant Pathogenic Fungi

DWI SURYANTO (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara)
NETTI IRAWATI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara)
ERMAN MUNIR (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara)



Article Info

Publish Date
12 Jan 2012

Abstract

A study on the isolation and characterization of chitinolytic bacteria and their potential to inhibit plant pathogenic fungi has been done. The bacteria were isolated from the soil of Karo, Langkat, and Bangka, Sumatra. Ganoderma boninense, Fusarium oxysporum, and Penicillium citrinum of the stock cultures in Laboratory of Microbiology Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara were used for growth inhibition assay by the isolated bacteria. KR05 and LK08 shared similar morphological and physiological characters; like wise, KR07 shared property similarities with BK08. All bacterial isolates inhibited the growth of G. boninense F. oxysporum , and P. citrinum at a different extent. LK08 showed the highest inhibition rate followed by BK07 and BK09. However, P. citrinum was inhibited more by BK07 and BK09. The crude enzyme preparation of the latter isolate exhibited the highest chitinase activity. The result suggested that their swarming activity seemed to contributed to inhibition of fungal growth.

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Journal Info

Abbrev

mionline

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, ...