Jurnal Ilmiah Pertanian
Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian

Isolasi, karakterisasi dan uji stabilitas pH bakteriofag Xanthomonas oryzae dari area persawahan

Desy Mar’atul Fadlilah (Department of Agrotechnology, Faculty of Agriculture and Business, Satya Wacana Christian University, Salatiga, Indonesia)
Andree Wijaya Setiawan (Department of Agrotechnology, Faculty of Agriculture and Business, Satya Wacana Christian University, Salatiga, Indonesia)
Yoga Aji Handoko (Department of Agrotechnology, Faculty of Agriculture and Business, Satya Wacana Christian University, Salatiga, Indonesia)



Article Info

Publish Date
24 Apr 2022

Abstract

The efforts to increase paddy yields are integrated with disease control. Bacterial Leaf Blight (BLB) is a disease that attacks paddy plants caused by Xanthomonas oryzae bacteria. So far, the control carried out by farmers is by spraying bactericides, planting disease-resistant varieties, and rotating plants that are not pathogenic hosts. However, this method of controlling has not obtained satisfactory results. Therefore, we need an alternative option in overcoming BLB disease, namely using bacteriophages as biocontrol agents in infecting Xanthomonas oryzae bacteria. This study explored the potential of bacteriophages isolated from soil samples near paddy plants' root areas and tested their particle's stability. The stages of experiments were soil sampling, isolation, purification, propagation, and pH stability examination. The results showed that the Xanthomonas oryzae bacteriophages had been isolated, purified, and propagated with the coding Kalibening 1 (XB1), Kalibening (XB2), and Kalibening 3 (XB3). The results of the pH stability test on the three samples also showed that the Xanthomonas oryzae bacteriophages experienced particle instability and the titer tended to drop in the pH range of 3-5, while the Xanthomonas oryzae bacteriophages tended to be stable at neutral and alkaline pH 7-11, particularly XB1 and XB2 bacteriophages.

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Journal Info

Abbrev

jip

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Ilmiah Pertanian (JIP) is a peer-reviewed, open-access journal that publishes original research articles regarding the advance of agriculture and land resources throughout the world. It publishes original scientific work related to strategic and applied studies in all aspects of agriculture, ...