Jurnal Riset Biologi dan Aplikasinya
Vol. 3 No. 1 (2021)

Screening of Extracellular Enzymes on Serratia marcescens strain MBC1

Meishy Handerlin Putri (Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia)
Kusuma Handayani (Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia)
Wawan A Setiawan (Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia)
Berliana Damayanti (Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia)
Cindy Lukyta Ratih (Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia)
Achmad Arifiyanto (Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia)



Article Info

Publish Date
31 Mar 2021

Abstract

Hydrolase enzymes are a class of enzymes used to break down the organic substrates into simpler molecules. Hydrolase enzymes play a role in biocontrol, industry, and public health. One of the hydrolase enzyme producers is bacteria. Serratia marcescens strain MBC1 is a collection in the Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung which enzymatic activities havent been known yet. This research aimed to determine the enzymatic activity of S. marcesescens strain MBC1 that can be used as a candidate for biocontrol agents, biosurfactant producers, industry, or in the health sector. The screening was carried out using a qualitative method plate assay using selective solid media for each test, with three replications for each test. The data obtained were analyzed statistically using analysis non-parametric kruskall-wallis test. The lipolytic activity of the S. marcescens strain MBC1 showed high efficacy with an enzymatic index of 5.52 and amylase, cellulases, protease, mannanase, and chitinase with enzymatic indexes, respectively, 4.17, 1.50, 0.69, 0.35, and 0.27. This enzyme activity of this isolate can be used as a candidate for biological control agents,biosurfactant producers, industry, or the health sector.

Copyrights © 2021






Journal Info

Abbrev

risetbiologi

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

Description

Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and ...