Str Locus Mutations In Paternity Case

Racy Youngest (Forensic Science Universitas Airlangga)
Vira Saamia (Forensic Laboratory Center)
Dwi Ana Oktaviani (Forensic Laboratory Center)
Setia Betaria Aritonang (Forensic Laboratory Center)
I Made Wiranatha (Forensic Laboratory Center)
Irfan Rofiq (Forensic Laboratory Center)

Jurnal Biosains Pascasarjana
Vol. 24 No. 1 (2022): VOL 24, NO 1 (2022): JURNAL BIOSAINS PASCASARJANA

DNA analysis is widely applied in solving forensic cases, especially Short Tandem Repat (STR) because of its advantages. In identifying the individual, the National Police compared the individual's DNA with that of his parents. Each anal has a pair of DNA fragments of which half are inherited by the father and the remainder by the mother according to Mendel's Law of Segregation. In this study, we compared DNA typing between the child and the mother with the help of PCR extracted by the Chelex method to find the mother fragment and obtain the father fragment. A child is the biological child of the alleged father if he or she has less than 2 exclusion STR loci. The results of this study revealed that all paternal fragments from the child were identical to the DNA fragments of the alleged father, except for one locus, namely CSF1PO which had a mutation. Mutations in the STR locus lower the paternity index, although it can still be concluded that the child is the biological child of the alleged father. Keywords: Paternity Test, DNA, STR, Mutation