ANNALES BOGORIENSES
Vol 25, No 1 (2021): ANNALES BOGORIENSES

Fermentation Tofu Using Consortium Lactic Acid Bacteria Isolated from Whey Tofu as Biocoagulant and Bioperservation

Siti Eka Yulianti (Pusat Penelitian Bioteknologi LIPI)



Article Info

Publish Date
15 Jun 2022

Abstract

Tofu is a traditional food that has high protein content and is prone to spoilage. A fermentation process with consortium lactic acid bacteria (LAB) that can produce bacteriocin has is carried out to get tofu that is more durable with better taste.  The Bacteria which are used in this study are Lactobacillus fermentum, Leuconostoc mesenteroides, and Lactobacillus plantarum.  L. fermentum is isolated from whey tofu. The antibacterial ability of each bacteria is measured by bacteriocins assay after incubation at 0 hours; 4 hours; and 8 hours in a row are L. fermentum 0; 2506.429 AU/mL; 1502.679 AU/mL;  L. mesenteroides 0 AU/mL; 2506.43 AU/mL; 2939.44 AU/mL; L. plantarum 760.39 AU/mL; 3341.25 AU/mL; 3889.29 AU/mL. Ratio inoculum bacteria (a:b:c) which are used are 2:1:1, 1:1:2, and 1:1:1 by using percentage of inoculum 10%. The Optimum inoculum age of these bacterium is 6 hours with µmax each bacteria in a row is 0.04 h-1, 0.02 h-1, dan 0.02 h-1. The fermentation is processed in 8 hours at 37ᵒC of temperature without agitation. From this optimation, the highest bacteriocin activity in tofu curd is 1767.857 AU/mL, which is in a ratio of 1:1:2 at the 4th hour.

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Journal Info

Abbrev

annales

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

Description

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as ...