Mediagro
Vol 9, No 2 (2013)

PENGKAJIAN VIABILITAS BENIH DENGAN TETRAZOLIUM TEST PADA JAGUNG DAN KEDELAI

Renan Subantoro (Fakultas Pertanian Universitas Wahid Hasyim Semarang)
Rossi Prabowo (Fakultas Pertanian Universitas Wahid Hasyim Semarang)



Article Info

Publish Date
19 Dec 2015

Abstract

Tetrazolium test is a test of dehydrogenase enzyme activity in seed tissue, so that the tissue is known to live or die in the embryo. The basic principle of this test is used chemical reduction of tetrazolium chloride 3,3,5 Triphenil initially colorless be red tissue. Staining intensity indicates the viability of the tissue. Tissue on seed dies, certainly not able to germinate seeds or germinated abnormally. This experiment using corn seed (monocots) and soybean seed (dicots). Testing seed viability quickand topography using tetrazolium salt solution and to control seed viability testing with germinated in germination plate. Both of these tests using a t-test. The results of the study are: corn seed viability testing quick by using the tetrazolium test can be conducted show significantly different results with the results of direct germination test (control), environmental factors on the test field with sand media should be controlled so that the optimum for germination, and seed are still viable indicated by a color change in the embryo and endosperm become red. . Key Words : Tetrazolium, Viability, Enzim Dehidrigenase, Seed

Copyrights © 2013






Journal Info

Abbrev

Mediagro

Publisher

Subject

Agriculture, Biological Sciences & Forestry Environmental Science

Description

The Journal Publishes in both print and online version. MEDIAGRO JOURNAL research paper in the field of Agribusiness, Agricultural Technology, Food Technology and general agricultural ...