Structural Elucidation of Secondary Metabolites in Sponge (Callyspongia pseudoreticulata) with N-Hexane Extract

M. Nurdin (Chemistry Department of Mathematic and Natural Science Faculty, Makassar Islamic University, South Sulawesi, 90245)
Hanapi Usman (Chemistry Department of Mathematic and Natural Science Faculty, Hasanuddin University, South Sulawesi, 90245)
Ambo Ala (Cultivation Department, Faculty of Agriculture Hasanuddin University Makassar, South Sulawesi, 90245)
Baharuddin Baharuddin (Plant Pests and Diseases Department, Faculty of Agriculture, Hasanuddin University, Makassar, South Sulawesi, 90245)

International Journal of Agriculture System
VOLUME 2 ISSUE 1, JUNE 2014

Sponge is one of the marine species that has a high bioactivity and contains secondary metabobolite compound which can be used as antibiotic and medicine. Terpenoid compound derivatives have been successfully isolated from sponge Callyspongia pseudoretilulata by a method in which the n-hexane extract weighing 11.28 g was fractionated using vacuum column chromatography with a non-polar eluent, n-hexane. The polarity of this eluent was improved by ethyl acetic resulting in fairly polar eluent, methanol. A total of 27 fractions were obtained and one of the compounds from n-hexane extract was structurally elucidated by NMR spectroscopy and two dimensions of homonuclear and heteronuclear (1H, 13C, DEPT, H-H COSY, HMQC, and HMBC). The identified compound was a hydrocarbon (1-ethyl cyclo hex cosana-1-amin) that indicated LC50 of 60.58 against A. salina and has a great potential as antitumor or anticancer.