Jurnal Kedokteran Gigi Terpadu
Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu

Aktivitas antioksidan ekstrak etanol rimpang curcuma xanthorrhiza roxb. Dan asam askorbat (Dengan Metode DPPH, ABTS, Dan NO)

Olivia Amanda Suwardi (Mahasiswa Fakultas Kedokteran Gigi, Universitas Trisakti)
Monica Dewi Ranggaini (Bagian Biologi Oral, Fakultas Kedokteran Gigi Universitas Trisakti)



Article Info

Publish Date
15 Aug 2022

Abstract

Background: Antioxidants are very important to prevent degenerative diseases. Natural ingredients used as natural antioxidants are C. xanthorrhiza Roxb. Purpose: To compare antioxidant activity between ethanol extract C. xanthorrhiza Roxb. and ascorbic acid with DPPH, ABTS, and NO methods. Methods: Experimental laboratory research. C. xanthorrhiza Roxb. was extracted by maceration method. In the DPPH, ABTS, and NO methods, C. xanthorrhiza Roxb. and ascorbic acid were incubated. Then the absorbance was measured at a wavelength 517 nm for the DPPH method, 745 nm for the ABTS method, and 516 nm for NO method. Results: In DPPH method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 194.74 g/mL and 0.29 g/mL for ascorbic acid, in ABTS method IC50 of ethanol extract C. xanthorrhiza Roxb. was 80.04 g/mL and 2.49 g/mL for ascorbic acid, in NO method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 164.11 g/mL and 0.54 g/mL for ascorbic acid. Conclusion: In DPPH and NO methods, C. xanthorrhiza Roxb. has weak antioxidant activity and in ABTS method has strong antioxidant activity. In DPPH, ABTS, and NO methods, ascorbic acid has very strong antioxidant activity.

Copyrights © 2022






Journal Info

Abbrev

jkgt

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology

Description

Jurnal Kedokteran Gigi Terpadu publishes articles and scientific work from Researches, Case Reports and Literature Reviews in Dentistry and Dental Science. The scopes vary from: Dental Surgery, Dental Forensics, Oral Biology, Oral Medicine, Dental Public Health and Preventive Dentistry, Paediatric ...