Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Vol. 7 No. 1 (2017)

Micropropagation of Rubus chrysophyllus Reinw. ex Miq. and Rubus fraxinifolius Poir.

Lily Ismaini (Cibodas Botanical Garden- Indonesian Institute of Sciences)
Destri Destri (Cibodas Botanical Garden- Indonesian Institute of Sciences)
Muhammad Imam Surya (Cibodas Botanical Garden- Indonesian Institute of Sciences)



Article Info

Publish Date
31 Jan 2017

Abstract

Rubus chrysophyllus and Rubus fraxinifolius are a native species in Indonesia, which has highly potential to be developed as fruits crops. Propagation is an important factor in developing a fruit cultivation. In vitro micropropagation is an important instrument to improve the quality of seedling. Our experiment was aimed to study the micropropagation of R. chrysophyllus and R. fraxinifolius. The shoot tips were cultured on MS medium supplemented with 10 mg/L indole-3-butyric acid (IBA) for enhancing roots of R. chrysophyllus and R. fraxinifolius in the in vitro condition. Then, the rooted plantlets were transplanted into cocopeat media for acclimatization. The results showed that R. chrysophyllus and R. fraxinifolius gave a different response on the medium contain 10 mg/L of IBA. The R. fraxinifolius shows the best results compared to R. chrysophyllus on 14 days after subculture. The roots length and roots numbers of R. fraxinifolius and R. chrysophyllus were 9.13 cm and 11.25; 2.20 cm and 2.00, respectively. Although R. fraxinifolius was growing better than R. chrysophyllus, but after acclimatization R. chrysophyllus were able to grow faster than R. fraxinifolius. Moreover, on the parameters of plant height shows that R. chrysophyllus (9.20 cm) were growth higher than R. fraxinifolius (4.05 cm) during acclimatization.

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Journal Info

Abbrev

jtrolis

Publisher

Subject

Agriculture, Biological Sciences & Forestry Environmental Science

Description

The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel ...