Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Vol. 11 No. 2 (2021)

Artificial propagation and embryonic growth in Stinging catfish, Heteropneustes fossilis (Bloch, 1794) using S-GnRHa (salmon gonadotropin releasing hormone analogue)

Tasrick Araf (Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, Bangladesh.)
Alal Hossain (Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, Bangladesh.)
Gourab Chowdhury (Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, Bangladesh.)
Mohammad Amzad Hossain (Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, Bangladesh.)
Md. Arifur Rahman (Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali, Bangladesh.)
Mohammed Mahbub Iqbal (Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, Bangladesh.)



Article Info

Publish Date
01 Jun 2021

Abstract

An experiment was performed to observe the potentiality of synthetic hormone analogue in artificial propagation i.e., the embryonic and larval development of Heteropneustes fossilis. Broodfish were injected with S-GnRHa according to the following concentration: 1, 2.5 and 5 ml kg-1 of body weight (BW) to females and 0.5, 1.125 and 2.5 ml kg-1 BW to males, in treatment groups T1, T2 and T3, respectively each with three replicates. The fishes were ovulated at about 10-11 hours after the hormone injection. Result showed the highest fertilization (83.11±1.36) and hatching rate (89.56±1.04) in T2, whereas ovulation rate was 100% in all treatment groups. First cleavage was observed in 30 minutes of post-fertilization. Embryonic developmental period sequentially for 2-cell, 4-cell, 8-cell, 16-cell, 32-cell, 64-cell, Morula, Blastula, Gastrula, somatic formation, yolk-plug, twisting movement and pre-hatching were 00:30, 00:45, 01:10, 01:30, 02:00, 02:30, 03:00, 04:00, 06:40, 09:00-18:00, 19:00, 20:00-21:00 and 22:00 hours, respectively. Hatching occurred after 23:00 hours of fertilization. Finally, the current result suggested that S-GnRHa might be an effective synthetic hormone in artificial propagation of H. fossilis.

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Journal Info

Abbrev

jtrolis

Publisher

Subject

Agriculture, Biological Sciences & Forestry Environmental Science

Description

The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel ...