Bioassay on n-Hexane Extract of Leaves Cassia alata against Candida albicans

Muhammad Bahi (Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala)
Radilla Mutia (Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala)
Mustanir Mustanir (Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala)
Endang Lukitaningsih (Fakultas Farmasi, Universitas Gadjah Mada, Jogjakarta)

Jurnal Natural
Volume 14, Number 1, March 2014

The study of biological activity from n-hexane extract of leaves’ Cassia alata had been done against Candida albicans as bioindicator. Dried leaves of C. alata (850 g) were macerated into n-hexane to obtain a dark green crude extract (31.79 g). The n-hexane crude extract was then subjected to vacuum liquid chromatography (VLC) and eluted with n-hexane and ethyl acetate to give 4 sub-fractions, namely fraction A (7.7251 g), fraction B (1.9419 g), fraction C (1.3565 g) and fraction D (1.4737 g) respectively. Based on phytochemical analysis, both nhexane crude extract and chromatographic sub-fractions contained steroids as their secondary metabolite constituent. The antifungal activity was tested against C. albicans using the agar-disc diffusion method with three different concentrations (10, 30 and 50%). Fraction D showed medium antifungal activity at 10.6, 11.3 and 12.6 of diameter-inhibition zones (mm) respectively.