Assessment of Tobacco Fields for the Prevalence of Verticillium dahliae in District Swabi, Pakistan

Tasiq Ahmad (Department of Biotechnology, University of Swabi, Pakistan)
Said Akram (Department of Biotechnology, University of Swabi, Pakistan)
Shah Fahad (Department of Biotechnology, University of Swabi, Pakistan)
Muhammad Ilyas (Department of Botany, Kohsar University Murree, Pakistan)
Mukhtar Alam (Department of Agriculture, University of Swabi, Pakistan)
Hazrat Hussain (Department of Biotechnology, University of Swabi, Pakistan)
Latifur Rehman (Department of Biotechnology, University of Swabi, Pakistan)

AGRIVITA, Journal of Agricultural Science
Vol 45, No 1 (2023)

Field and laboratory investigations of tobacco plants were carried out to analyze the distribution of Verticillium wilt caused by V. dahliae. V. dahliae is isolated from field Z (Tarkhai, Yarhussain region of Swabi). Species of Fusarium and Actinomycetes were detected in field B (Shaheedan, Yarhussain region of Swabi), while no V. dahliae was isolated from these fields. V. dahliae was identified based on its colony and conidia morphology. The colony was white, having oval spores and septate mycelium with phialides. The isolate also produced irregularly elongated dark brown to black color microsclerotia of various sizes. The spores count for V. dahliae isolate was 3.61 × 108 conidia/ml. The average colony diameter of V. dahliae after 9 days of incubation on PDA and CM media was 6.55 cm and 5.5 cm, respectively. The biology of Verticillium spp. in tobacco plants and soil in Swabi has not been studied. For the first time, this study reported the presence of V. dahliae in the tobacco fields of Swabi, KPK Pakistan. Detection and isolation of V. dahliae from field soil are necessary for studying the ecology and virulence and creating deterrent control strategies for Verticillium wilt diseases.