Journal of Global Pharma Technology
Volume 12 Issue 06 (2020) June 2020

Different Molecular Methods to Determine Genetic Diversity in Pseudomonas aeruginosa Clinical Isolates

Reem Akram Naji (Unknown)



Article Info

Publish Date
02 Jul 2020

Abstract

A total of (210) clinical specimens were collected from patients suffering from different infections such as burns, wounds, ear infections, diabetic foot, and urinary infections, cultured on differential media. All clinical specimens were cultured on different media. The results indicated that 22 (10 %) of the isolates were belong to P. aeruginosa. It was found that six isolates (27.27%) were isolated from wounds, five isolates (22.72%) from burns, six isolates (27.27%) from ear infections, two isolate (9%) from urine and three isolates (13.63%) from diabetic foot infections. In this study, used RAPD, BOX, and ERIC-PCR for the detection of phylogenetic diversity of pseudomonas aeruginosa isolated from different samples, In general, 18 isolates characterized using the three molecular techniques had comparable number of bands with some degree of polymorphism. P. aeruginosa isolates from the same source were clustered in to different groups. The three molecular techniques generated 2 main clusters, and the results of dendrogram of these techniques reveals that two isolates number(11,12) were similarity 100% in three methods techniques this suggest that this give the more discrimination power. While the pattern of others recorded some difference in the distribution. Keywords: Genotyping, Phenotyping, Pseudomonas aeruginosa, Molecular detection, RAPD, BOX, ERIC.

Copyrights © 2020






Journal Info

Abbrev

jgpt

Publisher

Subject

Medicine & Pharmacology

Description

ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and ...