Journal of Global Pharma Technology
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Using of Chlorella Vulgaris for Livestock Wastewater Treatment and the Expression of NR Gene

Nasrin Moshtaghi (Unknown)



Article Info

Publish Date
31 Aug 2017

Abstract

Algal treatment was performed for 30 days in three autoclaved livestock wastewaters. Nitrate concentrations in the three batch cultures and autoclaved livestock wastewaters were determined by spectrophotometer method. It was found that the capacity of Chlorella vulgaris removal was reached at rates of 89.22% nitrate in cattle livestock wastewater, 89.34% in sheep livestock wastewater and 89.41% in poultry livestock wastewater. Also, we studied the growth characteristics of C. vulgaris under three wastewater treatments within thirty days. The results showed that in the lag phase, the microalgae was treated in the wastewater with 501.33, 406.66, 381 mg. l-1 nitrate with a biomass productivity of   0.1, 0.09, 0.03g/l perday. On the other hand, we studied the removal of nitrite from wastewater by C. vulgaris. The results showed that the capacity of Chlorella vulgaris for nitrite removal reaches 87.50%, 43%, 65.15%, respectively. Moreover, the expression of nitrate reductase (NR)was analysed over six days in BG11 medium and livestock wastewater treatment. The highest fold of transcription was observed 96 hours after treatment in both of wastewater and culture medium. The transcript of gene at 96 hours in cattle wastewater was higher than BG11 culture medium. These result showed high potentials of Chlorella vulgaris to gene expression related to uptake of toxic nutrient and suitable candidate for gene isolation.Keyword: Algae, Livestock waste water, Nitrate reductase, Transcript.

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Journal Info

Abbrev

jgpt

Publisher

Subject

Medicine & Pharmacology

Description

ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and ...