Journal of Global Pharma Technology
Volume 11 Issue 09: (2019) September 2019

Ultrasound Stimulate Conjugation Between Wild Type and Genetically Engineered Agrobacterium tumefaciens and Sinorhizobiummeliloti

Raghad Nawaf Gergees Al-Zaidy (Unknown)



Article Info

Publish Date
16 Nov 2016

Abstract

Bacterial conjugations represent one of possibilities that transform bacteria. This study aimed to detect the occurrence of conjugation between two genera of bacteria includes wild strain A.tumefaciens (Atmco1), standard strain A. tumefaciens (C58Cl) and Sinorhizobiummeliloti. The results proved the conjugation through the sensitivity of those bacteria to specific antibiotics. The obtained data reported the stimulation of bacteria growth when they exposed to different ultrasound treatments. These ultrasound treatments include five period (0.0, 240, 480, 720 and 900) second for (47.6) KHZ. The treatments (240, 480) s increase colonies numbers between 25-72% when bacteria grown on the surface of the specific solid medium. The interesting results of this study are that the exposures of the conjugation mixture to the ultrasound treatment increase the Trans conjugant colonies between (23-80) % and increase the conjugation frequency twice. This may be probably as akey to enhance conjugation between those of bacteria species which are not able to conjugation.

Copyrights © 2019






Journal Info

Abbrev

jgpt

Publisher

Subject

Medicine & Pharmacology

Description

ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and ...