Makara Journal of Science
Vol. 16, No. 1

The Genetic Diversity of Endophytic and Phyllosphere Bacteria from Several Indonesian Herbal Plants

Soka, Yogiara Susan (Unknown)
Magdalena, Stella (Unknown)
Rachelia, Devi (Unknown)



Article Info

Publish Date
25 Apr 2012

Abstract

Herbal plants have been believed by Indonesians to be an alternative medicine to treat illnesses. The bioactive compounds in the plant can be derived from secondary metabolites or from endophytic and phyllosphere bacteria which coexist within medicinal plants. A total of 18 endophytic bacteria and 32 phyllosphere bacteria were isolated from the herbal plants of Citrus sp., Pluchea indica, Curcuma longa, Nothopanax scuttelarium, Piper crocatum, and Andrographis paniculata. About 72% of endophytic bacteria isolates have proteolytic activity and about 11% have lipolytic activity. On the other hand, about 59% of phyllosphere bacteria isolates have proteolytic activity and about 19% have lipolytic activity. Phylogenetic diversity analysis was conducted by using the amplified ribosomal DNA restriction analysis (ARDRA) method and the sequence of 16S rDNA was digested with endonuclease restriction enzymes: MspI, RsaI, and Sau961. The diversity of endophytic and phyllosphere bacterium from the samples of herbal plants was high. Bacteria isolated from the same herbal plant does not always have a close genetic relationship except for the bacteria isolated from the P. indica plant which showed a close genetic relationship with each other

Copyrights © 2012






Journal Info

Abbrev

publication:science

Publisher

Subject

Description

Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and ...