Majalah Kedokteran Andalas
Vol 46, No 2 (2023): Online April 2023

Diagnostics of RT-PCR Accuracy and GeneXpert Method on Covid-19 Examination

Seprina Zulaini (Magister Bioteknologi, Program Pascasarjana Universitas Andalas)



Article Info

Publish Date
11 Apr 2023

Abstract

The COVID-19 pandemic raises demands for significant diagnostics at all pre-analytical and post-analytical levels. At the beginning of the pandemic, molecular diagnostic tests were still limited and tended to take a long time. So far, the cases examined were using the Real-Time Polymerase Chain Reaction (RT-PCR) method. This method can be run on multiple samples and has a turnaround time of fewer than 4 hours. This study compared the accuracy of RT-PCR and GeneXpert Xpress assays in the Covid-19 test. This study examined 30 samples of suspected Covid-19 patients collected at the Central Laboratory of Diagnostic and Infectious Diseases Research, Andalas University, and the Laboratory of the West Sumatra Lung Hospital. The examination was carried out using the RT-PCR method and then GeneXpert with a cut-off value of 37.5 and 42.5, respectively. The results showed that the examination using GeneXpert had a sensitivity value of 96%, specificity of 100%, positive and negative predictive values of 100% and 83%, and accuracy of 480%. This proves that testing using GeneXpert has a high level of accuracy and can be used as a reliable alternative in diagnosing Covid-19 samples.

Copyrights © 2023






Journal Info

Abbrev

art

Publisher

Subject

Health Professions Medicine & Pharmacology Public Health

Description

Majalah Kedokteran Andalas (MKA) (p-ISSN: 0126-2092, e-ISSN: 2442-5230) is a peer-reviewed, open-access national journal published by Faculty of Medicine, Universitas Andalas and is dedicated to publish and disseminate research articles, literature reviews, and case reports, in the field of medicine ...