The aims of this study were to analyze the mediating effect of work motivation on the influence of job design and organizational culture on human resource performance. Research design is a detailed design that is used as a study guide that leads to research objectives. The object of this research is the human resources of the Karanganyar Regency Environmental Service. In this study there was a population of 55 people with a sample of 35 respondents. Sampling used a purposive sampling method, with the criteria of employees who have worked for more than one year and have at least a bachelor's degree. Test validity and reliability using smart PLS analysis on the outer model. Hypothesis testing uses the inner smart PLS model. The results of the analysis analysis show that there is a significant direct effect of work design on human resource performance and work motivation, there is a significant direct effect of organizational culture on work motivation and human resource performance, there is a significant direct effect between work motivation on human resource performance , there is an indirect effect of work design on human resource performance through work motivation mediating variables.
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