<![CDATA[Ecoenzyme liquid has benefits as a natural disinfectant. This study aimed to determine the antibacterial activity of ecoenzyme fluid made from papaya peel (Carica papaya L.), soursop skin (Annona muricata L.), neem leaf (Azadirachta indica) and lemongrass (Cymbopogon winterianus) against Escherichia coli bacteria isolated from dog skin in vitro. In this study, 4 concentrations of ecoenzymes were used, namely 25%, 50%, 75%, 100%, and 1 positive control (antibiotic Kanamycin) and 1 negative control. In this study, the research data were analyzed for variance and continued with the Games-Howell test. The results showed that the zones of inhibition on ecoenzymes with concentrations of 25%, 50%, 75%, and 100% were 0 ± 0, 0 ± 0, 8.42 ± 0.46, and 8.56 ± 0.96, respectively. positive was 20.95 ± 1.43 and negative control was 0 ± 0. The most effective ecoenzyme liquid inhibiting the growth of Escherichia coli in vitro was a concentration of 100%. It is necessary to do further research, namely the testing of ecoenzyme fluid against E. coli bacteria in vitro.]]>
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