Buletin Veteriner Udayana
Vol. 15 No. 4 August 2023

Uji Efektivitas Ekoenzim dalam Menghambat Pertumbuhan Jamur Curvularia Sp yang Diisolasi dari Kulit Anjing Secara In Vitro

Ni Putu Tiara Indriana (Mahasiswa Sarjana Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)
I Nyoman Suartha (Laboratorium Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)
Putu Henrywaesa Sudipa (Laboratorium Bakteriologi dan Mikologi, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)



Article Info

Publish Date
15 Feb 2023

Abstract

Dogs are very susceptible to infected by fungal species. The fungus that is often found infected dogs is Curvularia sp. Herbal medicine that is believed to be antifungal are ecoenzymes. The ecoenzymes in this study were made from papaya skin (Carica papaya L.), soursop (Annona muricata L.), neem leaves (Azadirachta indica A. Juss) and lemongrass (Cymbopogon nardus L.). This study was carried out to evaluate the effect of ecoenzymes against the growth of Curvularia sp used quantitative methode with modification of agar plate diffusion (Kirby Bauer) with well diffusion technique. This study was experimental and used a completely randomized design with 6 treatments, ecoenzyme concentration 5%, 10%, 15% and 100%, positive control used itraconazole, and negative control used aquades. Data analysis used ANOVA and continued with the Games-Howell test. The result showed that the inhibitory zones at ecoenzyme consentration 5%, 10%, 15%, and 100% were 3,50 mm; 4,82 mm; 6,02 mm; and 8,85 mm, respectively. Ecoenzyme in this study could inhibited fungal growth because of the ingredient contain antifungal substance like alkaloid carpaine, terpenoid, azadirachtin, nimbin, nimbidin, sitronela, and geraniol and pH reducer, so Curvularia spp that grew well in range of 4.0-10 could inhibited.

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