Journal Microbiology Science
Vol 1, No 1 (2021)

ISOLASI FUNGI ENDOFIT DAUN Srikaya (Annona muricata L.) SEBAGAI ANTIOKSIDAN SECARA KLT-AUTOGRAFI

Asri, Muhammad Ikhwan (Unknown)
Sabaruddin, Sabaruddin (Unknown)
Fitriana, Fitriana (Unknown)



Article Info

Publish Date
30 Sep 2021

Abstract

Antioxidants are compounds that can neutralize free radicals that produced by endophyte microbes. This study aims to isolate endophyte fungi and antioxidant activity of isolate endophyte fungi of Annona muricata L. leave used TLC-Autograph Method. The result of isolation endophyte fungi  Annona muricata L. leaves obtained 7 isolates namely FES1, FES2, FES3, FES4, FES5, FES6, dan FES7 colonies. Isolates of endophytic fungi were purified by quadran steak method to obtain pure isolates. The pure isolates obtained were fermented in Maltosa Yeast Broth (MYB) medium using shaker at 200 rpm for 7 x 24 hours to obtain secondary metabolites namely mycelia and supernatant. The results of testing the antioxidant activity of the supernatant extract of endophytic fungi isolates by TLC-Autograph using 0.04% DPPH spray reagent showed that the endophytic fungi isolate FES2 had free radical activity at Rf values of 0.94 and 0.87.

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Journal Info

Abbrev

microbiologyscience

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others ...