The anthracnose caused by the Colletotrichum acutatum Simmonds fungus is one of the limiting factors for the production of red chilli. The occurrence of anthracnose determined by the success of pathogenesis by C. acutatum. Also, red chilli which is resistant to anthracnose has a higher ascorbic acid content than vulnerable red chili. This paper presents scientific information about the ability to grow three C.acutatum isolates (Kulonprogo, Temanggung, and Pandeglang) on a culture medium given ascorbic acid. The three C. acutatum isolates have high pathogenicity. The results showed that administration of ascorbic acid in the culture medium only affected the dry weight of mycelium and did not affect the diameter length of the colony of C. acutatum.
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