ISOLASI MANGOSTIN DARI KULIT BUAH MANGGIS (Garcinia mangostana L.) DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP Bacilus cereus.

Sri Idawati (Politeknik Medica Farma Husada Mataram)

Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram
Vol. 4 No. 2 (2018): JURNAL PENELITIAN DAN KAJIAN ILMIAH KESEHATAN POLITEKNIK MEDICA FARMA HUSADA MA

One of the fruit plants that has the potential as a drug is mangosteen, especially on the skin of the fruit. The main bioactive compound and become a major compound in the mangosteen plant, namely ?-mangostin. This research has succeeded in isolating ?-mangostin through the extraction process using reflux method using ethanol 96%. Purification done by the recrystallization method with solvent ethanol:aquades (1:1). The result of extraction yield 29,93% and the result of purification obtained the yield of ?-mangostin from the weight of the extract was 2,81%. Initial identification of isolates using thin layer Chromatography (TLC) and melting point test. This yellow powder isolate has a melting point of 179,7 ºC. Next, structural elucidation is carried out using FT-IR spectroscopy data. Antibacterial activity test isolate compound (?-mangostin) of Bacilus cereus bacteria have the power inhibitory with MIC 0,031 µg/ml.