Correlation of Leucine-Rich-α-2-Glycoprotein- 1 (LRG-1) Level in Urine with Cervical Cancer Stage, Histology Type and Histology Grading

Prilly Astari (Departemen Obstetri dan Ginekologi Fakultas Kedokteran Hasanuddin University, Makassar)
Syahrul Rauf (Departemen Obstetri dan Ginekologi Fakultas Kedokteran Hasanuddin University, Makassar)
Elizabet C. Jusuf (Departemen Obstetri dan Ginekologi FK Hasanuddin University, Makassar)
St. Maisuri T Chalid (Departemen Obstetri dan Ginekologi Fakultas Kedokteran Hasanuddin University, Makassar)
Sharvianty Arifuddin (Departemen Obstetri dan Ginekologi Fakultas Kedokteran Hasanuddin University, Makassar)
Retno B Farid (Departemen Obstetri dan Ginekologi Fakultas Kedokteran Hasanuddin University, Makassar)

Indonesian Journal of Obstetrics & Gynecology Science
Volume 6 Nomor 2 Juli 2023

Objective: To determine if the level of LRG-1 in urine correlates with cervical cancer stage, histology type and histology gradingMethod: This cross-sectional study using ELISA to test urinary LRG-1 of 59 cervical cancer patients. Data were analyzed using Kruskal-Wallis test.Results: From the total of 59 samples, LRG-1 in urine ranged from 0.48 ng/mL to 170.43 ng/mL, with median value 58.42 ng/mL. A median value of 21.42±52.29 ng/mL was found in the urine at early stage and 115.32±59.36 ng/mL at advanced stage. Most patients had cervical cancer at advanced stage (69.4%), squamous cell carcinoma (66.1%), and grade cannot be assessed (45.8%). Median LRG-1 levels were highest in squamous cell carcinoma (66.42±60.89 ng/mL) and poorly differentiated (127.74 ±54.13 ng/mL). LRG-1 levels were significantly correlated with cervical cancer stage (p-value=0.045) but not histological type (p-value=0.940) or histopathological grade (p-value=0.488).Conclusion: The more advanced the cervical cancer stage, the more elevated urinary LRG-1 levels. LRG-1 contributes to angiogenesis and antiapoptotic processes in cancer. Further studies are required to identify and evaluate LRG-1 in urine as an important biomarker for making clinical decisions and developing potential treatments.Hubungan Kadar Protein Leucine-Rich-α-2-Glycoprotein-1 (LRG-1) Urine dengan Stadium, Tipe Histologis, dan Derajat Diferensiasi Kanker ServiksAbstrakTujuan: Untuk mengetahui hubungan kadar LRG-1 dalam urine dengan stadium, tipe histologis, dan derajat diferensiasi kanker serviks.Metode: Studi ini menggunakan desain penelitian cross-sectional pada 59 perempuan yang telah didiagnosis kanker serviks, kemudian diperiksa kadar protein LRG-1 dalam urine dengan metode ELISA. Uji statistik menggunakan Kruskal Wallis. Hasil: Dari total 59 sampel didapatkan kadar LRG-1 dalam urine terendah 0,48 ng/mL dan tertinggi 170,43 ng/mL, nilai median 58,42 ng/mL. Nilai median pada stadium awal 21,42±52,29 ng/mL dan stadium lanjut 115,32±59,36 ng/mL. Lebih banyak penderita mengalami kanker serviks pada stadium lanjut (69,4%), tipe histopatologis Squamous Cell Carcinoma (66,1%), derajat diferensiasi tidak dapat ditentukan (45,8%). Median tertinggi kadar LRG-1 pada tipe Squamous Cell Carcinoma (66,42±60,89 ng/mL), dan derajat diferensiasi yang buruk (127,74±54,13 ng/mL). Terdapat hubungan yang signifikan antara kadar LRG-1 dan stadium kanker serviks (nilai p = 0,045), tetapi tidak dengan tipe histologis (nilai p=0,940) dan derajat diferensiasi (nilai p=0,488).Kesimpulan: Semakin tinggi stadium maka semakin tinggi kadar protein LRG-1 dalam urine. LRG-1 berperan dalam proses angiogenesis dan antiapoptosis pada kanker. Diperlukan penelitian lebih lanjut agar identifikasi dan evaluasi biomarker LRG-1 urine dapat menjadi penanda penting yang membantu dalam pengambilan keputusan klinis, serta pengembangan terapi.Kata Kunci: Derajat diferensiasi, Kanker serviks, LRG-1, Stadium, Tipe histologis