Chiral Separation of Econazole by High Performance Liquid Chromatography Method using Cyclodextrin as Chiral Column

Dadan Hermawan (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Indonesia)
Cacu Cacu (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman)
Khansa Salsabila (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman)
Suwandri Suwandri (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman)
Amin Fatoni (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman)
Uyi Sulaeman (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman)
Ponco Iswanto (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman)
Mudasir Mudasir (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada)
Hassan Y. Aboul-Enein (Department of Pharmaceutical and Medicinal Chemistry, Pharmaceutical and Drug Industries Research Division, National Research Center (NRC) Dokki)

Molekul: Jurnal Ilmiah Kimia
Vol 17 No 2 (2022)

The chiral separation of econazole, an antifungal drug with one chiral center has been successfully carried out using the high-performance liquid chromatography (HPLC) method. Enantioresolution of econazole (Rs = 2.29) was achieved using cyclodextrin-based chiral column (Astec Cyclobond, 25 cm × 4.6 mm × 5 μm), mobile phase composition of acetonitrile : water (0.2% HCOOH) (20:80, v/v), and UV detection of 220 nm.The optimized HPLC method has been applied for the quantitative determination of econazole in the pharmaceutical (liquid) sample withpercentage recovery of 100.75 % (RSD = 0,95%; n = 3). The effect of several HPLC parameters on the chiral separation of econazole was also evaluated and the method was successfully validated in terms of linearity, accuracy, precision, and selectivity. The present HPLC method was simple, short analysis time, and high resolution.