Phosphorylated Ataxia Telangiectasia Mutated (pATM) Enzyme-Linked Immunosorbent Assay (ELISA) for Predicting Radiation Induces Normal Tissue Toxicity in Radiotherapy Patients: A Systematic Review

Sofiati Purnami (1. Master Program in Biology, Department of Biology, Universitas Nasional, Jakarta 2. Research Center for Safety, Metrology, and Nuclear Quality Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jakarta)
Viria Agesti Suvifan (Directorate of Strengthening and Partnership of Research and Innovation Infrastructure, Deputy for Research and Innovation Infrastructure, National Research and Innovation Agency, Jakarta)
Dwi Ramadhani (Research Center for Radioisotope, Radiopharmaceutical, and Biodosimetry Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jakarta)
Yanti Lusiyanti (Research Center for Safety, Metrology, and Nuclear Quality Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jakarta)
Darlina Darlina (Research Center for Safety, Metrology, and Nuclear Quality Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jakarta)
Nastiti Rahajeng (Directorate of Strengthening and Partnership of Research and Innovation Infrastructure, Deputy for Research and Innovation Infrastructure, National Research and Innovation Agency, Jakarta)
Mukh Syaifudin (Research Center for Radioisotope, Radiopharmaceutical, and Biodosimetry Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Jakarta)
Dwi Ari Pujianto (Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta)
Retno Widowati (Department of Biology, Graduate School, Universitas Nasional, Jakarta,)

Indonesian Journal of Cancer
Vol 17, No 3 (2023): September

Background: An adverse normal tissue response, such as normal tissue toxicity (NTT), is present in radiotherapy (RT) patients and can limit the effectiveness of the RT treatment. Identifying patients with adverse tissue responses before RT had clinical benefits and individual radiosensitivity (IRS) is considered an important factor in NTT incidences. Therefore, this systematic study aimed to determine the possibility of using phosphorylated Ataxia Telangiectasia Mutated (pATM) Enzyme-Linked Immunosorbent Assay (ELISA) to predict NTT in RT patients.Methods: A comprehensive data search was conducted in three electronic databases, namely PUBMED CENTRAL, ScienceDirect, and SCOPUS. The quality of relevant publications was independently evaluated using the PICO (participants, intervention/exposure, comparison, and outcome) approach.Results: : A total of 47 articles were retrieved, 41 of which were assessed based on the titles and abstracts. Furthermore, 39 articles were excluded, and 2 were included in this study.Conclusions: The phosphorylated ATM ELISA on lymphocytes showed promising results for IRS prediction in RT patients. However, these assumptions should be validated on a larger RT patient cohort.