Studi Pendahuluan Pengembangan Vaksin DNA Menggunakan Gen Nukleoprotein Virus SARS-COV-2

Ni Putu Sutrisna Dewi (Program Studi Magister, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, 80225, Indonesia)
I Gusti Ngurah Kade Mahardika (Laboratorium Biomedik dan Biologi Molekuler Fakultas Kedokteran Hewan, Universitas Udayana, Jl. Raya Sesetan Gang Markisa 6A, Denpasar, Bali, 80223, Indonesia)
Ida Bagus Kade Suardana (Laboratorium Virologi Fakultas Kedokteran Hewan, Universitas Udayana Jl. PB. Sudirman, Denpasar, Bali, 80225, Indonesia)

Buletin Veteriner Udayana
Vol. 15 No. 5 October 2023

DNA vaccines have become an interesting area of research in the fight against infectious diseases in animals and humans. DNA vaccines are vaccine formulations that use DNA fragments as antigens. The DNA in vaccines contains genetic information extracted from the disease agent, such as a virus or bacteria, that it is trying to target. This research aims to develop DNA vaccines in animals and humans. We developed plasmid DNA clones with the Covid-19 virus nucleoprotein gene that was transformed into E. coli stellar bacteria. The vaccine was injected in mice. The antibody was tested using an Enzyme-linked immunosorbent assay with purified recombinant NP protein. The results of the study showed that a clone of plasmid DNA with the nucleoprotein gene of the SARS-Cov-2 virus could be developed and proven. Administration of plasmid DNA clones with nucleoprotein genes in mice could induce the formation of specific antibodies against the nucleoprotein of the SARS-Cov-2 virus. The antibodies were increased from week 1 to weeks 4 and 5 then decreased in week 6 post-vaccination.