Synthesis and Characterization of Silver Nanoparticles (AgNPs) using Chemico-physical Methods

Tooba Siddiqui (Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India)
Mohammad Khalid Zia (Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India)
Mohammad Muaz (Interdisciplinary Nanotechnology Centre, Aligarh Muslim University, India)
Haseeb Ahsan (Department of Biochemistry, Faculty of Dentistry, Jamia Millia Islamia, India)
Fahim Halim Khan (Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India)

Indonesian Journal of Chemical Analysis (IJCA)
Vol. 6 No. 2 (2023): Indonesian Journal of Chemical Analysis

Silver nanoparticles (AgNPs) have several applications in nanoscience and nanomedicine such as antibacterial, antifungal, anti-inflammatory and anti-angiogenic properties. In the present study, the AgNPs were synthesized by the chemical method and characterised using a combination of UV-Visible (UV-Vis) spectrophotometry, Fourier Transform Infra-Red (FTIR) spectroscopy, transmission electron microscopy (TEM) and X-ray diffraction (XRD). The AgNPs were made by chemically reducing AgNO3 with NaBH4 leading to the formation of a characteristic yellowish-brown colour of AgNPs. To investigate the formation, crystalline behavior, and quality of AgNPs powder, XRD measurements were performed.  The produced AgNPs were found to be spherical, dispersed, and scattered in form and the particle size of the AgNPs formed was about 15 nm and they were crystalline in nature. The interaction of AgNO3 with biomolecules may have numerous applications in nanoscience and nanomedicine.