AGRIVITA, Journal of Agricultural Science
Vol 38, No 1 (2016): FEBRUARY

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

M.K. Sabullah (Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia)
M.F. Rahman (Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia)
S.A. Ahmad (Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia)
M.R. Sulaiman (School of Food Science and Nutrition, Universiti Malaysia Sabah, Malaysia)
M.S. Shukor (Snoc International Sdn Bhd, Lot 343, Jalan 7/16 Kawasan Perindustrian Nilai 7, Inland Port, 71800, Negeri Sembilan, Malaysia)
N.A. Shamaan (Faculty of Medicine and Health Sciences, Islamic Science University of Malaysia, 55100 Kuala Lumpur, Malaysia)
M.Y. Shukor (Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia)



Article Info

Publish Date
14 Jan 2016

Abstract

Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.

Copyrights © 2016






Journal Info

Abbrev

AGRIVITA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research ...