HAYATI Journal of Biosciences
Vol. 31 No. 1 (2024): January 2024

Identification, Characterization and Antioxidant Activity of Yellowish-Orange Pigments Actinobacteria

Farhana Halimah Rusyda (Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia)
Irmanida Batubara (Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia. Tropical Biopharmaca Research Center, IPB University, Bogor 16128, Indonesia)
Yulin Lestari (Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia. Tropical Biopharmaca Research Center, IPB University, Bogor 16128, Indonesia)



Article Info

Publish Date
02 Nov 2023

Abstract

Actinobacteria are Gram-positive bacteria that can produce various secondary metabolites, including pigments that have potential as antioxidants. This study assessed the characteristics of yellowish-orange pigmented actinobacteria. The antioxidant activity of their extract pigment was tested by DPPH and analyzed by LC-MS. The identification results based on the 16S rRNA gene showed that the HRA isolate had 99.91% similarity value to Streptomyces gramineus JR-43, the HVA isolate had 99.22% similarities value to S. tendae ATCC 19812 and the HVB isolate had 81.18% similarities value to Rhodococcus ruber DSM 43338. Meanwhile, AGM 2.2 and 2.3 isolates had similarities to S. xanthophaeus NRRL B-5414 with similarity values of 98.95% and 99.82%, respectively. The Inhibitory Concentration (IC50) of actinobacterial crude extract pigments ranges from 53.38 µg/ml to 184.38 µg/ml. The HVB isolates with the major compound luteolin, have the highest antioxidant activity. The crude extract pigment of HVB isolates may consider rich in luteolin and has potency as an antioxidant.

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Journal Info

Abbrev

hayati

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...