Grimsa Journal of Science Engineering and Technology
Vol. 1 No. 2 (2023): December 2023

Hippobroma longiflora L Leaves as a Natural Inhibitor of Cataract Progression: A Comprehensive Study Integrating Ethanol Extract, HPLC, and Molecular Docking Approaches

Eva Imelda (Department of Ophthalmology, School of Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
Department of Ophthalmology, General Hospital Dr. Zainoel Abidin, Banda Aceh 23126, Indonesia)

Ulfa Fitria (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Ulmiza Putri Mutia (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Syahrul Syahrul (Department of Neurology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Masitha Dewi Sari (Department of Ophtalmology, Medical Faculty, North Sumatera University, Medan, 20222, Indonesia)
Sarra Mutiara Adev (Medical Program, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia)
Alya Mutiara Adev (Faculty of Medicine, Muhammadiyah University of North Sumatra, Medan, 20238, Indonesia)
Zakiaturrahmi Zakiaturrahmi (Department of Physiology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Navneet Shamsundar Toshniwal (Director Navneet Hospital, Solapur, Maharashtra, India)



Article Info

Publish Date
27 Nov 2023

Abstract

Cataract remains the highest preventable cause of blindness in the world. The H. longiflora plant (Hippobroma longiflora L) is a plant that has empirically been widely used to treat a variety of eye diseases including cataract. This plant has high antioxidant activity due to its high phenolic and flavonoid content. The H. longiflora plant was extracted using an ethanol solvent and its compound content was analyzed using GCMS and phytochemistry analysis. The extracts obtained were tested with TPC, TFC, antioxidants and quercetine compound with HPLC. From phytochemical and GCMS analysis, it was found that triterpenoid compounds were not found in the sample, and the compound 1-Isobutyl-2,5-dimethyl-4-phenyl-piperidin-4-ol was the most abundant (37.77%), as well as 4-Penten-1-ol, 5-phenyl-3-pperidino (1,30%) were the least abundant in the ethanol extract of the H. longiflora leaves extract. The total phenolic, flavonoid and HPLC results of quercetin were 139.21 mg GAE/g; 11.48 mg QE/G and 0.009 mg/G. Meanwhile, in an antioxidant activity test, it was found that the ethanol extract of the H. longiflora leaves has very strong antioxidants. This finding revealed the potential of ethanol extracts of H. longiflora leaves as a cataract inhibitor through its process as an antioxidant.

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Journal Info

Abbrev

gjset

Publisher

Subject

Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Engineering Medicine & Pharmacology Physics

Description

Grimsa Journal of Science Engineering and Technology aims to provide a platform for researchers, scholars, and professionals to share their innovative ideas, findings, and insights in the following areas: Science and Innovation, Engineering and Technology, Information Technology and Computer ...