Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering)
Vol 12, No 4 (2023): December 2023

Use of Portable Fluorescence Spectroscopy and SIMCA Method to Test The Authenticity of Apis mellifera Honey From Coffee Flower Nectar Mixed With Two Artificial Sweeteners

Sapto Kuncoro (Department of Agricultural Engineering, The University of Lampung)
Diding Suhandy (Universitas Lampung)
Warji Warji (Universitas Lampung)
Dicky Ervandi (Universitas Lampung)



Article Info

Publish Date
02 Dec 2023

Abstract

Honey with coffee flowers nectar is native honey formed from flower nectar. In this investigation, corn syrup and rice syrup, two artificial sweeteners, were utilized as an adulterant. Portable fluorescence spectroscopy and the SIMCA method are the tools and techniques employed. There were up to 20 samples of pure Apis mellifera honey and up to 120 samples of mixed honey (MC), each used twice. Data on the emission spectra, which are excited at a wavelength of 365 nm, were measured over the wavelength range of 300-800 nm. To improve accuracy, sensitivity, and specificity, the original spectral data was altered using a number of pre-treatments. Pretreatment with the original data with smoothing moving average may accurately identify samples and provides 100% accuracy, sensitivity, and specificity. One of the steps of the SIMCA approach, the cumulative PC, has a value of 92%, which indicates that it well explains the variation of the data. The x-loading plot's values are near the peak of the waves at 378 and 460 nm, indicating the existence of phenolic and flavonoid chemicals at those wavelengths. Keywords:   Apis mellifera honey, Corn syrup, Rice syrup, Portable Spectroscopy, SIMCA.

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Journal Info

Abbrev

JTP

Publisher

Subject

Agriculture, Biological Sciences & Forestry Engineering

Description

Jurnal Teknik Pertanian Lampung or Journal of Agricultural Engineering (JTEP-L) is a peer-reviewed open-access journal. The journal invites scientists and engineers throughout the world to exchange and disseminate theoretical and practice-oriented researches in the whole aspect of Agricultural ...