Double step method in lipid extraction from biomass Aurantiochytrium sp powder

Endah Sulistiawati (Universitas Ahmad Dahlan)
Suhendra Suhendra (Universitas Ahmad Dahlan)
Martomo Setyawan (Universitas Ahmad Dahlan)
Anis Herliyati Mahsunah (National Research and Inovation Agency)

International Journal of Advances in Applied Sciences
Vol 12, No 4: December 2023

Aurantiochytrium sp is a marine microalgae that is rich in lipids. Extraction of lipids from microalgae requires effort to select a suitable solvent and extraction method. This research used a double-step extraction method to study a mixture of n-hexane and methanol as a solvent. The variables studied were stirring time, the n-hexane to methanol (H/M) mixture ratio, and the solvent-to-biomass ratio (S/B). This research concluded that an optimum stirring time was 30 min, and a mixture of n-hexane and methanol solvents with a volume ratio of 1:1 is optimum. The optimum solvent-to-biomass ratio was S/B=20 mL/g dry microalgae. Under these conditions, the yield of oil was 83.88%. Double-step extraction can increase the yield by 10-40%.