Buletin Veteriner Udayana
Vol. 15 No. 6 December 2023

Perbandingan Teknik Mini-Flotac dan Teknik Flotasi dalam Uji Koproskopis Cacing pada Feses Penyu Hijau

Nurul Audy Baiqhaq Fathonah (Mahasiswa Program Sarjana Kedokteran Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)
Ida Bagus Windia Adnyana (Laboratorium Patologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia)
Ida Ayu Pasti Apsari (Laboratorium Parasitologi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234)



Article Info

Publish Date
15 Jul 2023

Abstract

The green turtle (Chelonia mydas) as a protected animal is an indicator of the health of marine ecosystems because it is a sentinel species. The most common cause of green turtle death is endoparasite infection. The discovery of worm eggs is based on coproscopic techniques using sedimentation, desorption, centrifugation and flotation of material. The Mini-FLOTAC technique is a direct method that was recently developed from the previous flotation technique for the diagnosis of intestinal parasitic infections. This study aims to compare the results of examination of worm eggs using the Mini-FLOTAC Technique and the Flotation Technique. The study used green turtle feces at the TCEC Serangan Bali Conservation and Education Center. The sample used 2 grams for each type of technique. Furthermore, an examination was carried out with the Mini-FLOTAC Technique and Flotation Technique in the Turtle Conservation and Education Center TCEC Serangan, Bali laboratory. The results were then analyzed with manual calculations and assistance from an online statistical calculator. The value of the k parameter is 0, interprets that there is a significant difference between the Mini-FLOTAC Technique and the Flotation Technique. This interpretation is supported by the results of the sensitivity values of the Mini-FLOTAC Technique reaching 100% and the Touch Flotation Technique 0% which indicates a significant difference between the results of examination of worm eggs based on their taxonomy on the Mini-FLOTAC Technique and the Flotation Technique. The Mini-FLOTAC technique showed superior effectiveness compared to the Flotation Technique for detecting worm eggs in this study. Clearer vision of eggs is sometimes seen in the results of the Flotation Technique. Making a filter for Fill-Flotac at 250µm for green turtle (Chelonia mydas) examination in order to produce clearer results.

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