Jurnal Medika Veterinaria
Vol 10, No 2 (2016): J. Med. Vet.

15. KONSENTRASI PROTEIN ANTIGEN EKSKRETORI/SEKRETORI DAN SOMATIK PADA Fasciola gigantica DAN Eurytrema pancreaticum The Protein Concentration of Excretory/Secretory and Somatic Antigen from Fasciola gigantica and Eurytrema pancreaticum

Muhammad Hambal (Laboratorium Parasitologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh)



Article Info

Publish Date
01 May 2016

Abstract

The study was aimed at finding out the difference of protein content between excretory/secretory and somatic antigen of Fasciola gigantica as well as between excretory/secretory of Fasciola gigantica and Eurytrema pancreaticum. As many as 32 Fasciola gigantica and 30 Eurytrema pancreaticum were placed into 80 ml Roswell Park Memorial Institute (RPMI) 1640 and incubated for 6 hours. Excretory/secretory as well as somatic antigen was isolated and the concentration of protein was determined, using Lowry method. The result revealed the different of protein concentration. The concentrations of excretory/secretory antigen protein in Fasciola gigantica and Eurytrema pancreaticum were 3.386 mg/ml and 0.128, mg/ml respectively. The concentrations of somatic protein and Fasciola gigantica and Eurytrema pancreaticum were 8.028 mg/ml and 0.534 mg/ml, respectively. It can be concluded that the protein concentration from somatic tissue of Fasciola gigantica and Eurytrema pancreaticum is higher than the protein concentration from excretory/secretory of Fasciola gigantica and Eurytrema pancreaticum. The protein concentration from excerory/secretory antigen of Fasciola gigantica is higher than the protein concentration from excretory/secretory antigen of Eurytrema pancreaticum. ____________________________________________________________________________________________________________________ Key words: Fasciola gigantica, Eurytrema pancreaticum, protein concentation

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Journal Info

Abbrev

JMV

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

Jurnal Medika Veterinaria is published biannually in February and August. The scope of this journal includes Veterinary Medicine, Animal Science, Science, and Medicine. The journal accepts original research papers, case study, and review article. All submissions are evaluated by peer reviewers who ...