Isolasi heparin dari jeroan ikan tuna (Thunnus sp.) : Isolation of heparin from tuna viscera (Thunnus sp.)

Safrina Dyah Hardiningtyas (Departemen Teknologi Hasil Perikanan, IPB University)
Hanin Bela Kartika (Departemen Teknologi Hasil Perikanan, IPB University)
Kustiariyah Tarman (Departemen Teknologi Hasil Perikanan, IPB University)

Jurnal Pengolahan Hasil Perikanan Indonesia
Vol 26 No 3 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26 (3)

Tuna is a valuable commodity in Indonesia and its production value has been increasing annually. Tuna processing produces 50-70% waste. The use of viscera waste in the production of novel biomaterials, including heparin, has been documented. The objective of this study was to obtain heparin from tuna viscera, specifically from the liver, stomach, intestine, and pyloric caeca. Heparin extraction was accomplished using an enzymatic technique utilizing papain. Crude heparin extract was subjected to acetone fractionation for further purification. The functional groups of pure heparin were analyzed using a Fourier Transform Infrared (FTIR) spectrophotometer, while the heparin content was determined using a sulfate glycosaminoglycan (GAG) assay. The findings of the study indicated that the intestinal and pyloric caeca had a heparin content of 43% and yielded 0.66±0.06%. Pure heparin tuna viscera comprise various functional groups, including carboxyl, acetyl, hydroxyl, ester cycles, and sulfated N atoms. This research indicates that tuna viscera has the potential to serve as a substitute for heparin, a commonly utilized medical resource.