In Vitro Test of Leilem Leaf Ethanol Activity on β-Glucosidase Enzyme Inhibition

Krispela Trifosa Oktavia Siregar (Program Studi Pendidikan Dokter, Fakultas Kedokteran, Universitas Sam Ratulangi, Manado, Indonesia)
Fona Dwiana Hermina Budiarso (Bagian Kimia, Fakultas Kedokteran, Universitas Sam Ratulangi, Manado, Indonesia)
Billy Johnson Kepel (Bagian Kimia, Fakultas Kedokteran, Universitas Sam Ratulangi, Manado, Indonesia)
Fatimawali Fatimawali (Bagian Kimia, Fakultas Kedokteran, Universitas Sam Ratulangi, Manado, Indonesia)
Aaltje Ellen Manampiring (Bagian Kimia, Fakultas Kedokteran, Universitas Sam Ratulangi, Manado, Indonesia)
Widdhi Bodhi (Bagian Kimia, Fakultas Kedokteran, Universitas Sam Ratulangi, Manado, Indonesia)

Jurnal Ilmiah Sains
Volume 24 Issue 1, April 2024

Diabetes is a chronic metabolic disease with signs of elevated blood glucose levels. One of the targets of antidiabetic drugs is to inhibit the enzyme α-/β-glucosidase which works in the process of glucose absorption. Leilem plant (Clerodendrum minahassae Teisjm. & Binn.) is well known and used as a dish and medicine for several diseases in Minahasa and has potential as an antidiabetic agent. This study aimed to determine the activity of ethanolic extract of leilem leaves in inhibiting β-glucosidase enzyme. The extract was prepared with 96% ethanol. Antidiabetic properties were measured by β-glucosidase enzyme inhibitory activity. The inhibitory concentration (IC50) values of the extract compared tp acarbose were 28,229 and 13,716. Ethanol extract of leilem leaves inhibits β-glucosidase enzyme. Keywords : β-glucosidase; ethanol extract; in vitro; leilem leaf