Jurnal Teknik Kimia
Vol 30 No 1 (2024): Jurnal Teknik Kimia

Hydrolysis profile of gadung (dioscorea hispida dennst) starch to glucose using alpha amylase enzyme

Utari Agustina (Department of Chemical Engineering, State Polytechnic of Sriwijaya, Palembang, Indonesia)
Abu Hasan (Department of Chemical Engineering, State Polytechnic of Sriwijaya, Palembang, Indonesia)
Indah Purnamasari (Department of Chemical Engineering, State Polytechnic of Sriwijaya, Palembang, Indonesia)



Article Info

Publish Date
31 Mar 2024

Abstract

Indonesia is an agrarian Country that has abundant natural resources. It has potential to be used as industrial raw materials such as sugar reduction from starch. Gadung, which is abundant in Gandus and it can be monitored for its presence in the dry season, is one source of starch. The aims of this research is to obtain glucose with a high yield percentage, to know the optimum concentration of α-amylase enzyme to hydrolysis gadung starch into glucose, and to know the relationship between hydrolysis time and concentration the glucose formed by the addition of the enzyme variation. The implementation of the research is carried out in three stages, first is preparation of raw materials, hydrolysis of gadung starch into glucose enzymatically, and analysis of glucose level using the UV-Vis spectrophotometry with Nelson reaction. The free variables used are the time variation of hydrolysis 0, 10, 20, 30, 40, 50 minutes and alpha amylase enzyme 1 %, 2 %, 3 % b/v. The optimum hydrolysis results on this study were at 1 % b/v enzyme variation, 10 minutes hydrolysis time, and a temperature of 90 oC. The yield of glucose concentration is 255.35 ppm. From this reseach, it is known that the longer the starch hydrolysis takes place, the observed glucose tends to experience in fluctuative consentration caused by the increase of α- amylase enzyme variations.

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Journal Info

Abbrev

jtk

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Industrial & Manufacturing Engineering

Description

Jurnal Teknik Kimia merupakan publikasi tulisan ilmiah hasil riset dan pengalaman lapangan di bidang Teknik Kimia, mulai dari prinsip dasar atau fundamental sampai pada penerapan/aplikasinya di industri. Jurnal Teknik Kimia dalam versi cetak telah diterbitkan sejak tahun 1996. Jurnal Teknik Kimia ...