Lymphatic Filariasis is a chronic infectious disease that remains a public health problem in Indonesia as it can cause permanent disability and is prevalent in most parts of the country. In 2023, 7,995 chronic cases were recorded. This survey aimed to assess filariasis transmission in Ambon City, specifically to determine the microfilariae rate (Mf rate). This research is a quantitative study with a cross-sectional design. This activity also evaluates the Mass Filariasis Disease Eradication Program (POPM) implemented in Ambon City in five years, from 2015 to 2019. The population in this survey was Ambon City, which was over 18 years old, with a total of 249,608 people. The sample consisted of 1,230 respondents from 30 clusters representing five sub-districts in Ambon City. Sample selection was randomized using the Survey Sampler Builder (SSB) application. The survey results showed that the Mf rate of Ambon City at stage 1 of the Transmission Assessment Survey (TAS) was 0.08%, with only one positive slide out of 1,230 samples, which was below the threshold. In conclusion, filariasis transmission in Ambon City has been successfully reduced. It is recommended that the Ambon City Health Office provide treatment to respondents identified as positive and continue to control infectious vectors in the area.
                        
                        
                        
                        
                            
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