Food ScienTech Journal
Vol 6, No 1 (2024)

Characterization and Determination of pH, Alcohol, and Temperature Tolerance of Microorganisms Recovered From Vegetable Samples

Onajobi, Ismail Babatunde (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Samson, Oyindamola John (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Adekola, Hafeez Aderinsayo (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Aina, Suliamon Adebisi (Department of Zoology and Environmental Biology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Salisu, Titilola Fausat (Department of Zoology and Environmental Biology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Kazeem, Muinat Olanike (Department of Microbiology, Faculty of Science, University of Ilorin, Ilorin, Kwara State, Nigeria)
Ogunlana, Inumidun Esther (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Adetunji, Faidat Ayomide (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Nubi, Mary Rereloluwa (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Salau, Raliat Omobolanle (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)
Adebajo, Lawrence Olubukunade (Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria)



Article Info

Publish Date
03 Jul 2024

Abstract

This study aims to investigate the spectrum of bacteria and yeasts present in soaked vegetables and their resilience to varying pH levels, alcohol, and temperatures. Four samples were collected from Ago-Iwoye market, and initial isolation and identification were conducted using streaking techniques and biochemical tests. Standard procedures were employed to assess bacteria and yeast tolerance to different pH levels, alcohol concentrations, and temperature ranges. The biochemical tests identified microorganisms, including Lactobacillus spp., Lactobacillus spp., Enterococcus spp., Leuconostoc spp., Escherichia coli, and Saccharomyces cerevisiae. Bacterial and yeast isolates demonstrated viability at both 10°C and 45°C, while no visible growth occurred at 60°C, indicating the respective heat resistance of these microorganisms commonly found in vegetable samples. The evaluation of microbial tolerance to varying pH conditions is pivotal for exploring adaptability in food processing and digestion, particularly in fermentation processes. The research concludes the microbial diversity present, distinguishing between beneficial and potentially harmful strains.

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Journal Info

Abbrev

fsj

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Immunology & microbiology

Description

FSJ is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of Food Technology, with particular attention paid to the exploration and development of natural products derived from tropical—and especially Indonesian—biodiversity. ...