Jurnal AgroBiogen
Vol 5, No 2 (2009): Oktober

Analisis Keragaman Genetik Acremonium yang Berasosiasi dengan Tanaman Gaharu Menggunakan Teknik Random Amplified Polymorphic DNA (RAPD)

Dewi Rahmawati (Laboratorium Bioteknologi, SEAMEO BIOTROP, Jl. Raya Tajur Km 6, Bogor)
Nurita Toruan-Mathius (Plant Production and Biotechnology Div., TCLZ and Biotechnologi Lab., PT SMART Tbk, Jl. Raya Pajajaran 78 F-6, Bogor)



Article Info

Publish Date
23 Aug 2016

Abstract

Agarwoodor gaharu is a plant that has a high economic value in Asia,due to its use for production of incense and traditionalmedicines. The agarwood formation occurs in the trunk androots of trees that have been infected by a fungus, such asAcremonium spp. Various fungi were associated with theagarwood formation. Acremonium is generally considered ashighly polyphyletic, contains distantly related fungi. A studywas done to identify genetic diversities in 10 isolates ofAcremonium spp. from four different areas in Indonesia thatare associated with Aquilaria and Gyrinops verstigii using theRandom Amplified Polymorphic DNA (RAPD) technique.Eight RAPD primers, i.e., OPA 02, OPB 04, OPB 07, OPB 17,OPC 11, OPD 03, OPD 05, and OPE 07 were used in theanalyses. The results indicated that similarity index values ofthe genetic variation ranged from 0.21 to 0.97. Based on theNei and Li’s similarity coefficients, these values indicatingthe presence of high degree of genetic variability. The lowestdegree of genetic similarity were found between isolates F(Acremonium spp., which is associated with G. verstigii fromMataram, Nusa Tenggara Barat), and LM2 from south coastalarea of West Sumatra. The highest genetic similarity value(0.97) was found between isolates Sr2 and Sr4 from Sorong,Papua. Results from the cluster analysis indicated that theisolates could be grouped into two major clusters that wereassociated with their geographical locations.

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Journal Info

Abbrev

JAB

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya ...