Sidik Jari DNA 88 Plasma Nutfah Ubi Jalar di Indonesia Berdasarkan Delapan Penanda SSR

Nurul Hidayatun (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. [phone] Faks. [phone])
Chaerani Chaerani (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. [phone] Faks. [phone])
Dwinita Wikan Utami (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. [phone] Faks. [phone])

Jurnal AgroBiogen
Vol 7, No 2 (2011): Oktober

DNA Fingerprinting of Indonesian 88 Sweet PotatoGermplasm Based on Eight SSR Markers. NurulHidayatun, Chaerani, and Dwinita W. Utami. Indonesiapossesses a great number of sweet potato varieties.Understanding the diversity and distribution of this geneticresource is essential for its management and future use. Theobjective of this study was to elaborate the molecularcharacter as DNA finger print of Indonesian sweet potatogermplasm. Eight fluorescent labeled SSR primers wereused to amplify DNA of 88 sweet potato accessionsconsisting of improved varieties and landraces collectedfrom 7 islands in Indonesia. The amplified products weredetected using capillary electrophoresis method in CEQGenetic Analysis System machine. A total of 135 allelesranging from 8 to 36 alleles per locus with an average of 17alleles were generated. Each accession had a uniquemicrosatellite finger print marked by specific combination of11 to 22 alleles in 8 SSR loci. Dendrogram generated byUPGMA based on simple matching coefficients produced 4nonspecific groups at 80% similarity. The groups revealedthe possibilities that the accessions were distributed fromsimilar genetic resources.