Jurnal AgroBiogen
Vol 5, No 2 (2009): Oktober

Hubungan Kekerabatan Jamur Pelapuk Putih Pleurotus spp. dengan Analisis Isoenzim

Achmad Achmad (Staf Laboratorium Perlindungan Hutan, Departemen Silvikultur Fakultas Kehutanan Institut Pertanian Bogor, Jl. Lingkar Akademik Kampus IPB Darmaga, Kotak Pos 168, Bogor 16680)
Elis N. Herliyana (Staf Laboratorium Perlindungan Hutan, Departemen Silvikultur Fakultas Kehutanan Institut Pertanian Bogor, Jl. Lingkar Akademik Kampus IPB Darmaga, Kotak Pos 168, Bogor 16680)
Ficky R. Agustian (Alumni Fakultas Kehutanan Institut Pertanian Bogor)



Article Info

Publish Date
23 Aug 2016

Abstract

Pleurotus spp. is anedible mushroom commonly found on stem of wide leaftrees or other wooden plants in the forest. A number ofPleurotus species are found in Indonesia, and some of themhave been cultivated. An accurate technique is needed toidentify the species of Pleurotus correctly; one of the methodis the isoenzyme technique. Apart from its simplicity, cheapnessand quickness, this method also gives accurate informationon phylogenetic relationship among the Pleurotusspp. The technique was used to determine phylogeneticrelationship in 6 isolates of Pleurotus spp., i.e., Pleurotussp.8, Pleurotus sp.6, Pleurotus sp.1, Pleurotus sp.7, andPleurotus sp.9, using the GOT System. Results of the analysisindicated that all the Pleurotus isolates tested produced twobands with similar thickness, except for Pleurotus sp.1 thatproduced one band that move to the cathode (-). Anotherisolate of Pleurotus spp. produced bands tend to move to theanode (+). The genetic distances between Pleurotus sp.8was similar to that of Pleurotus sp.9, while that of Pleurotussp.6 was similar to Pleurotus sp.7. Genetic distances ofPleurotus sp.8 or Pleurotus sp.9 was similar to Pleurotus sp.6or Pleurotus sp.7, with the longest distance on Pleurotussp.1. Pleurotus sp.1 showed a different migration distance,where one of the isoenzym band tend to move to thecathode (-). This indicated that Pleurotus sp.1 has differentphylogenetic relationship with the other Pleurotus spp.

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Journal Info

Abbrev

JAB

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya ...