Variasi Genetik Jeruk Keprok SoE (Citrus reticulata Blanco) Hasil Radiasi Sinar Gamma Menggunakan Penanda ISSR

Farida Yulianti (Balai Penelitian Tanaman Jeruk dan Buah Subtropika, Jl. Raya Tlekung Junrejo, Batu, Kotak Pos 22, Malang 65301 Telp. [phone] Faks. [phone])
C. Martasari (Balai Penelitian Tanaman Jeruk dan Buah Subtropika, Jl. Raya Tlekung Junrejo, Batu, Kotak Pos 22, Malang 65301 Telp. [phone] Faks. [phone])
nFN Karsinah (Balai Penelitian Tanaman Buah Tropika, Jl. Raya Solok Aripan Km. 8, PO Box. 5, Solok 2730-Sumatera Barat Telp. (0755) 20137 Faks. (0755) 20592)
Tangguh Hartanto (Universitas Brawijaya, Jl. Mayjen Haryono 169, Lowokwaru, Malang 65145, Jawa Timur Telp. [phone], [phone] Faks. [phone])

Buletin Plasma Nutfah
Vol 16, No 2 (2010): December

Genetic Variation of Keprok SoE Mandarin (Citrus reticulata Blanco) Resulted from Gamma Iradiation Based on ISSR Markers. SoE mandarin is one of the best mandarin from Indonesia which has been chosen as one of mandarin for import substitution. The citrus quality could be improved through breeding program, one of this program was mutation breeding using gamma rays irradiation. The research was aimed to obtain information of SoE mandarin genetic variation derived from gamma rays irradiation using ISSR marker. The research was conducted at Breeding and Tissue Culture Laboratory of Indonesian Citrus and Subtropical Research Institute (ICISFRI) from May to September 2008. Five ISSR primers were used to amplify DNA samples. Matrix data was counted and dendrogram of samples was established using UPGMA and SAHN methods. The resulst showed that 3 of the primers indicated polymorphism. About 22 locus were amplified from 3 primers, 9 (40.9%) locus showed polymorphism. The genetic similarity of SoE mandarin derived from gamma rays irradiation were 73-100%. AbstrakJeruk keprok SoE merupakan salah satu jeruk keprok unggulan Indonesia untuk mensubstitusi jeruk impor. Kualitas jeruk dapat ditingkatkan melalui program pemuliaan, salah satunya adalah melalui pemuliaan mutasi dengan menggunakan sinar gamma. Tujuan penelitian ini adalah untuk mendapatkan informasi tentang variasi genetik jeruk keprok SoE hasil radiasi sinar gamma menggunakan penanda ISSR. Penelitian dilaksanakan di Laboratorium Pemuliaan dan Kultur Jaringan, Balai Penelitian Tanaman Jeruk dan Buah Subtropika (Balitjestro) pada bulan Mei-September 2008. Lima penanda ISSR digunakan untuk mengamplifikasi sampel DNA. Pengelompokan tanaman di dalam dendrogram dihitung menurut UPGMA menggunakan metode SAHN. Hasil penelitian menunjukkan bahwa tiga penanda mengindikasikan adanya polimorfisme. Dari 22 lokus yang terbentuk dari tiga penanda, sembilan lokus (40,9%) menunjukkan polimorfisme. Tingkat kesamaan genetik jeruk keprok SoE hasil radiasi sinar gamma berkisar antara 73-100%.