Karakterisasi dan Identifikasi Isolat Bakteri Endofitik Penghambat Jamur Patogen Padi

Yadi Suryadi (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. [phone] Faks. [phone])
Tri P. Priyatno (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. [phone] Faks. [phone])
I Made Samudra (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. [phone] Faks. [phone])
Dwi N. Susilowati (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. [phone] Faks. [phone])
Patricia Patricia (Jurusan Biologi Fakultas Sains dan Matematika UPH-Karawaci Tangerang)
Wahyu Irawati (Jurusan Biologi Fakultas Sains dan Matematika UPH-Karawaci Tangerang)

Buletin Plasma Nutfah
Vol 19, No 1 (2013): June

Disease caused byfungal pathogens often causing damage on rice crop. Thisstudy was aimed to characterize 10 endohytic bacterial isolatesin suppression of rice pathogenic fungi. Characterization by invitro test showed several endophytic isolates effective againstfungal pathogen Rhizoctonia solani (Rs) and Pyriculariaoryzae (Po). The bacterial culture filtrates could inhibit radialgrowth of fungal colonies with the Rs ranged percentageinhibition of 32.9-99.4%, whilst inhibition against Po wereranged from 3-98.2%, respectively. Based on chitinase assay,it was indicated that gram negative bacteria of E 76 isolateproduced clear zone and highest chitinolytic index. Theanalysis to the base sequence (total 1,322 bp) using 16s rRNAgene sequencing revealed that E76 isolates had 99% similaritywith Burkholderia sp.