<![CDATA[This study aimed to evaluate the decreasing methane gas production in sheep by supplementing Moringa oleifera extract in the ration. The study was conducted in a Completely Randomized Design with One Way Classification. The treatment of Moringa oleifera extract was offered at 0, 0.25 and 0.50% of DM ration, each with six replicates, which constitutes 18 treatment units. The materials included ruminal fluid of three freshly slaughtered thin-tailed sheep derived from an abattoir in Banyumas Central Java. Moringa oleifera leaves were oven-dried at 60ºC for 2 x 24 h, ground into powder, and extracted using ethanol. The ration consisted of concentrate and ammoniated rice straw with a ratio of 60:40 (DM basis). The concentrate was composed of 2:1 rice bran and coconut meal. The measured variables included total protozoa, methane gas production, total bacteria, N-NH3 concentration, and protein synthesis of ruminal fluid. The result indicated 0.50% Moringa oleifera extract (DM basis) produced the lowest total protozoa, the highest total bacteria and protein microbe synthesis, and the lowest methane gas production.]]>
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